Cloning: Advanced Techniques in rDNA technology

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Date/Time
Date(s) - 12/11/2018 - 15/11/2018
10:30 am - 2:30 pm

Location
Central Dogma Pvt Ltd

Categories No Categories


Topics Covered

  • Isolation of expression vector
  • Amplification of the gene by PCR (Theory on Primer design)
  • Digestion of vector and PCR product by restriction enzymes
  • Preparation of competent cells
  • Ligation and transformation into competent E.coli host
  • Selection of clones by colony PCR

Key Feature: Complete Hands-on Training; Certificate will be given after successful completion

Contact: Dr Anjali; 9890038204